Into the chromosome cuatro, all about three QTLs was in fact at the 66
Box plots of land proving the results of your own marker genotypic classes (AAAA, AAAB, AABB, ABBB, BBBB) out-of 4 applicant SNPs toward tuber glucose attention (GLU); bud-avoid fry colour (FBE); stem-tuber fry color (FSE); ID, Idaho
Brand new co-localization away from numerous QTLs escort review Chicago IL in the maturity locus area within studies is actually consistent with the observed activities off phenotypic correlations. 01) with lots of attributes overlapping a comparable genetic reputation (Fig. 1). By way of example, EBID11 and VWID11 scores both exhibited a strong bad correlation with MAT95ID and MAT120ID. Which trend can get give an explanation for biggest impression QTL which have later readiness closely regarding the defer onset of VW and you may EB, or simply, early maturity resulting in early start of VW and you may EB. A relatively high relationship was also seen ranging from vine maturity and progress practice, that have early readiness of the so much more dispersed increases practice. Alternatively, a decreased relationship (r ? 0.2) anywhere between vine maturity and you can one another tuber sugar quantity and you can bud-end fry color was in keeping with different types with the QTL consequences.
Tuber sugar, bud-prevent and you will stem-end fry colour
Along with chromosome 5, significant QTLs to own sugar, bud-prevent and you may base-stop fry colour was basically seen into the overlapping aspects of chromosomes cuatro, six, 10, and eleven. 0 cM with the LOD scores ranging from cuatro.five to six.0 (Table step 3, Fig. 4). Such QTLs explained ranging from seven.dos and you may eleven.3% of the trait difference. Research of various easy habits conveyed a single most readily useful-fitting design for everyone about three qualities, with a dual-simplex allele (AAAB ? AAAB) to your homologous chromosomes H2 and you can H5 out of RGR and you may Public relations, respectively (Table 3, A lot more file 8: Figure S4). For sugar, the new B allele is in the large sugar focus, when you’re having bud-stop and you may stalk-prevent fry colour, the current presence of the B allele indicated all the way down reflectance viewpoints (Fig. 3b).
LOD rating shipments away from QTLs for a few traits over the 12 potato chromosomes (Chr). Abbreviations is actually as with Fig. step one
A candidate SNP for a double-simplex configuration on H2 and H5 is solcap_c2_12976 at position 67.4 cM. On the reference PGSC v4.03 Pseudomolecules, this SNP maps to the long arm of chromosome 4 at position 69.1 Mb on the F-box and WD40 domain protein gene (PGSC0003DMG400003339), which is close (< 2 Mb) to several genes associated with starch/sugar inter-conversion as annotated in the potato reference genome, and/or associated with tuber quality traits . They include alpha amylase (AMY-4/1 PGSC0003DMG400007974, AMY-4/2 PGSC0003DMG400009891), hexose transporter (HT-4/3, PGSC0003DMG400009994), invertase (NV-4, PGSC0003DMG400009936), and starch branching enzyme I (SBE I, PGSC0003DMG400009981).
On chromosome 6, significant QTLs mapped at 53 cM for glucose and at 43 cM for FBE and SBE, respectively. The LOD profiles ranged from 5.4 to 7.6 and explained between 10.8 and 16.1% of the trait variance, respectively (Table 3, Additional file 9: Figure S5). The best model for all three traits was a double-simplex configuration with allele effects on H2 and H5. As in chromosome 4, the presence of the B alleles was associated with an increase in glucose concentration and a decrease in reflectance readings (Fig. 3c). There are four candidate SNPs with a double simplex configuration nearby (solcap_snp_c2_33777, solcap_snp_c1_10130, solcap_snp_c2_5769, solcap_snp_c2_56145, c1_3003). All were significantly (P-value < 0.0001) associated with the mean trait values, however, solcap_snp_c2_56145 had on average the highest percent of variance explained (9% to 16%). The SNP solcap_snp_c2_56145 maps at position 51.4 Mb of the potato reference genome on the ATP binding protein gene PGSC0003DMG400033089. This SNP is located in a region (
seven Mb) which has multiple genes of starch/glucose inter-sales and hexokinase (HXK-six, PGSC0003DMG400016521), fructokinase (FRK-6/step one, PGSC0003DMG400027017, FRK-6/2, PGSC0003DMG400026916), and you can invertase (INV-na-6/step 1, PGSC0003DMG400026107, INV-6/2, PGSC0003DMG400033142) genes.